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Despite these obvious advantages, Sal-T measurements have not gained general acceptance in the clinical and research communities, largely due to uncertainties over the accuracy and reliability of the T assays (9, 10). Sal-T was stable at ambient temperature for up to 5 days, after freeze-thawing and 3 years frozen storage. Saliva (200 μL) was extracted with 1 mL of methyl-tert-butyl ether following the addition of D5-testosterone.
Testosterone in blood is either free (unbound) or bound. Saliva testing is easy to perform at home. So, I spent several weeks finding relevant research papers and finally consulted my medical friend for a professional opinion. This section collects any data citations, data availability statements, or supplementary materials included in this article. We wish to thank the patients and personnel of the Dialysis Unit at the Military Institute of Medicine-National Research Institute in Warsaw involved in the creation of this cohort. For this reason, we did not test the differences in testosterone concentrations between CKD G3 and CKD G4 groups.

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